Yeast extract solution for cell-free protein synthesis, method for preparation thereof and method for cell-free protein synthesis using same
US7387884B2 · kind B2 · utility
20Cited by
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4Claims
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Key dates
| Filing date | Jan 7, 2004 |
| Grant date | Jun 17, 2008 |
| Priority date | — |
| Expiry date | Oct 24, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N1/063
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a preparation method of a yeast extract solution for cell-free protein synthesis, which solution is easy to prepare and is capable of synthesizing a higher amount of a protein than by conventional yeast extract solutions, the yeast extract solution, a cell-free synthesis method of protein, which uses the yeast extract solution, and a kit for cell-free protein synthesis containing the yeast extract solution. The method of the present invention includes rupturing a yeast cell in a frozen state, and obtaining an extract thereof.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.