Method for construction and use of Kluyveromyces lactis promoter variants in K. lactis that substantially lack E. coli transcriptional capability
US7390636B2 · kind B2 · utility
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16Claims
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Key dates
| Filing date | Apr 8, 2005 |
| Grant date | Jun 24, 2008 |
| Priority date | — |
| Expiry date | Dec 7, 2025 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2820/702
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods and compositions are provided relating to production of recombinant protein in yeast. A modified PLAC4 is described where one or more mutations may be introduced into the Pribnow box-like sequences in the promoter. The modified promoter when placed upstream of a target gene in a vector causes a significant reduction of target gene expression in transformed bacteria but produces efficient expression of the target gene in yeast.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.