System and method for obtaining and maintaining high-resistance seals in patch clamp recordings

US7390650B2 · kind B2 · utility

Assignee

• Cellectricon AB · SE

Inventors

• Mattias Karlsson · Huskvarna, SE
• Owe Orwar · Hagfors, SE
• Daniel T. Chiu · Seattle, US
• Jon Sinclair · Handen, SE
• Kent Jardemark · Tyresö, SE
• Jessica Olofsson · Handen, SE
• Johan Pihl · Handen, SE
• Cecilia Farre · Kungsängen, SE

Key dates

• Filing date: Aug 20, 2003
• Grant date: Jun 24, 2008
• Expiry date: Jun 27, 2025

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/48728
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention provides a system, system components, and a method for rapidly obtaining and stably maintaining a cell in optimal contact with the cell-contacting surface of a sensor in a cell-based biosensor. In one aspect, the system maximizes the seal between a whole cell and the cell-contact surface of a patch clamp micropipette, maximizing the efficiency of a whole cell patch clamp recording.