Signalling compounds and methods for detecting hydrogen peroxide
US7390670B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 20, 2003 |
| Grant date | Jun 24, 2008 |
| Priority date | — |
| Expiry date | Dec 18, 2023 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/206664
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods and compound useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula:is reacted with peroxide. Sig is a non-polymeric organic group, B is a boron atom, and each R is independently selected from hydrogen, alkyl and aryl groups and can be joined together as a straight or branched alkylene chain forming a ring or as an aromatic ring. A detectable product compound of the formula Sig—OH or Sig—O− is produced and detected by measuring color, absorbance, fluorescence, chemiluminescence, or bioluminescence. The signalling compound itself does not possess the detectable property or does so only to a very weak degree. The methods can be used as a detectable signal in assays for peroxide or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.