Methods and compositions for interaction trap assays
US7393318B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 16, 2004 |
| Grant date | Jul 1, 2008 |
| Priority date | — |
| Expiry date | Jul 14, 2025 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/5008
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides methods and compositions for interaction trap assays for detecting protein-protein, protein-DNA, or protein-RNA interactions. The methods and compositions of the invention may also be used to identify agents which may agonize or antagonize a protein-protein, protein-DNA, or protein-RNA interaction. In certain embodiments, the interaction trap system of the invention is useful for screening libraries with greater than 107 members. In other embodiments, the interaction trap system of the invention is used in conjunction with flow cytometry. The invention further provides a means for simultaneously screening a target protein or nucleic acid sequence for the ability to interact with two or more test proteins or nucleic acids.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.