Use of multiple recombination sites with unique specificity in recombinational cloning

US7393632B2 · kind B2 · utility

Assignee

• Invitrogen Corporation · US

Inventors

• David Cheo · Kensington, US
• Michael A. Brasch · Gaithersburg, US
• Gary Temple · Washington Grove, US
• James L. Hartley · Frederick, US
• Devon Byrd · Germantown, US

Key dates

• Filing date: Dec 11, 2000
• Grant date: Jul 1, 2008
• Expiry date: Sep 10, 2021

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/66
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites with unique specificity. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. Such molecules and/or compounds or combinations of such molecules and/or compounds can also be bound through recombination to various structures or supports according to the invention.