Terminal-phosphate-labeled nucleotides with new linkers
US7393640B2 · kind B2 · utility
86Cited by
3References
10Claims
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Key dates
| Filing date | Feb 5, 2004 |
| Grant date | Jul 1, 2008 |
| Priority date | — |
| Expiry date | Jun 21, 2025 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P19/34
- WIPO fieldBasic materials chemistry
- WIPO sectorChemistry
Abstract
The present invention describes methods of using terminal-phosphate-labeled nucleotides in the presence of a manganese salt to enhance their substrate properties towards various enzymes. Particularly described are methods of detecting a nucleic acid in a sample, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases, in the presence of a manganese salt. Further provided are manganese complexes of terminal-phosphate-labeled nucleotides as well as terminal-phosphate-labeled nucleotides with new linkers with enhanced substrate properties.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.