Method of preparing a chromatography matrix

US7396467B2 · kind B2 · utility

Assignee

• GE Healthcare Bio-Science AB · US

Inventors

• Hans Berg · Uppsala, SE
• David Buckley · Rochester, US
• Maria Brannholm · Uppsala, SE
• Anders Hagvall · Uppsala, SE
• Henrik Ihre · Uppsala, SE
• Eva Holmgren · Uppsala, SE
• Anders Larsson · Uppsala, SE
• Dag Lindstrom

Key dates

• Filing date: Oct 13, 2004
• Grant date: Jul 8, 2008
• Expiry date: Mar 8, 2025

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01J2220/4825
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

The present invention relates to a method of preparing a porous cross-linked polysaccharide chromatography matrix, which comprises to provide an aqueous solution of a gellable polysaccharide, wherein part of the hydroxyl groups are substituted with groups which are not susceptible to nucleophilic attack; to provide essentially spherical droplets of the substituted polysaccharide solution; to form a gel of the substituted polysaccharide solution; and to cross-link the gel by adding a cross-linking agent. The invention also encompasses a chromatography column packed with a matrix so produced as well as use thereof in e.g. protein purification.