Method of adjusting the working range of a multi-analyte assay
US7396689B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 6, 2005 |
| Grant date | Jul 8, 2008 |
| Priority date | — |
| Expiry date | Sep 9, 2025 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/5306
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention features a method of adjusting the concentration of at least one but not all of a plurality of analytes in a fluid sample to match a known working range of detection of an analyte assay system, where each of the plurality of analytes may or may not be present within an expected initial concentration range having a high end and a low end, and at least one analyte has a high end expected concentration range that exceeds the high end of the working range of the assay system. The expected concentration of the high concentration analyte is adjusted by a proportional scaling constant, α, so that the high end of the adjusted expected concentration range is less than or equal to the high end of the working range, without adjusting the expected concentration range of at least one other of the plurality of analytes. Adjustment is preferably accomplished by adding to the solution phase of the assay one or more scaling agents, each scaling agent binding with specificity to an analyte and thereby preventing it from being detected by the assay system, e.g., by competing with binding to immobilized capture agent. This scaling method contrasts with prior methods, in which a concentra…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.