Recombinase polymerase amplification
US7399590B2 · kind B2 · utility
210Cited by
7References
190Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Sep 1, 2004 |
| Grant date | Jul 15, 2008 |
| Priority date | — |
| Expiry date | Sep 29, 2025 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/70
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This disclosure describe three related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes. Further, the improved processivity of the disclosed methods may allow amplification of DNA up to hundreds of megabases in length.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.