Assay for inhibitors of ispH

US7402408B2 · kind B2 · utility

Assignee

• Adelbert Bacher · DE

Inventors

• Adelbert Bacher · Garching bei München, DE
• Petra Adam · Neustadt an der Aisch, DE
• Wolfgang Eisenreich · Garching bei München, DE
• Tobias Gräwert · Augsburg, DE
• Stefan Hecht · Bad Aibling, DE
• Felix Rohdich · Freising, DE
• Ferdinand Zepeck · Kundl, AT

Key dates

• Filing date: Jan 15, 2004
• Grant date: Jul 22, 2008
• Expiry date: Oct 23, 2024

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/26
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Assay for testing a sample for the presence or absence of inhibition of the enzymatic conversion of 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate into isopentenyl diphosphate and/or dimethylallyl diphosphate by the following steps: (a) reacting an aqueous mixture containing 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate, a 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphnte reductase, and a reducing agent under predetermined reaction conditions for a predetermined period of time; (b) analyzing the reaction mixture obtained in step (a) for the consumed amount of 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate and/or NAD(P)H and/or for the produced amount of isopentenyl diphosphate, and/or dimethylallyl diphosphate and/or NAD(P)+; (c) repeating step (a) in the presence of the sample to be tested; (d) repeating step (b) with the reaction mixture defined in step (c); (e) comparing the results of steps (b) and (d).