Patent · US Expired

Methods and kits for identifying scavengers of reactive oxygen species (ROS)

US7405081B2 · kind B2 · utility

4Cited by
8References
14Claims
0Family size

Inventor

Key dates

Filing dateFeb 5, 2002
Grant dateJul 29, 2008
Priority date—
Expiry dateFeb 5, 2022

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG01N33/5008
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

This invention relates to methods and kits for determining the level of H2O2 inside a cell, and for determining whether a test compound has ability to scavenge a reactive oxygen species (ROS). The methods and diagnostic kits of this invention employ a cell containing a promoter which is inducible by an ROS, such as the H2O2 -inducible KatA promoter of Agrobacterium tumefaciens. The methods of this invention may also be used to select for new or improved ROS scavengers by expressing a library of test scavengers in cells which express a reporter from a ROS-inducible promoter and selecting for those cells whose level of ROS-inducible expression of the reporter is reduced.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.