Analytical method using particles and test kit for performing the method
US7405084B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 30, 1998 |
| Grant date | Jul 29, 2008 |
| Priority date | — |
| Expiry date | Jul 23, 2022 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/97
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for use in a flow matrix, which utilizes biospecific affinity reactions to detect an analyte in the sample, and which comprises allowing the sample comprising the analyte and an analytically detectable reactant (Reactant*) to migrate through flow channels in a flow matrix to a detection zone located in the matrix, in which there is a firmly anchored biospecific affinity reactant (Capturer), and capturing the Reactant* in the detection zone in an amount related to the amount of analyte in the sample. The Reactant* has labeled particles of an analytically detectable group, and the Capturer is anchored to the matrix by immobilized particles which exhibit hydrophilic groups on their surface. A test kit comprises a flow matrix having a detection zone in which there is a firmly anchored biospecific affinity reactant (Capturer), and an analytically detectable reactant (Reactant*). The Reactant* has labeled particles of an analytically detectable group, and the Capturer is anchored to the matrix by immobilized particles which exhibit hydrophilic groups on their surface.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.