Skin substitutes with improved barrier function
US7407805B2 · kind B2 · utility
Inventors
Key dates
| Filing date | Sep 27, 2005 |
| Grant date | Aug 5, 2008 |
| Priority date | — |
| Expiry date | Mar 5, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/54
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to in vitro cultured skin substitutes, and in particular to in vitro cultured skin substitutes that have improved barrier function. In some embodiments, improved barrier function is a result of improved culture conditions, while in other embodiments, improved barrier function results from genetic modification of keratinocytes. Improved culture conditions to improve barrier function include organotypic culture in the presence of linoleic acid and/or linoleic acid at about 75% humidity. Suitable genetic modifications for improving barrier function includes transfection with a DNA construct capable of expressing GKLF.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.