Patent · US Expired

B12 -dependent dehydratases with improved reaction kinetics

US7410754B1 · kind B1 · utility

2Cited by

1References

3Claims

0Family size

Assignee

E.I. DU PONT DE NEMOURS AND COMPANY · US

Inventors

Katharine J. Gibson · Wilmington, US
Der-Ing Liao · Wilmington, US
Xiao-Song Tang · Hockessin, US

Key dates

Filing date	Dec 16, 2003
Grant date	Aug 12, 2008
Priority date	—
Expiry date	Aug 29, 2024

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N9/88
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Sequences of B12-dependent dehydratases with improved reaction kinetics are presented. Use of these B12-dependent dehydratases reduce the rate of the enzyme's suicide inactivation in the presence of glycerol and 1,3-propanediol. The enzymes were created using error-prone PCR and oligonucleotide-directed mutagenesis to target the DhaB1 gene, which encodes the α-subunit of glycerol dehydratase. Mutants with improved reaction kinetics were rapidly identified using high throughput assays.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.