Method for making recombinant peptides or proteins using soluble endoproteases
US7410775B2 · kind B2 · utility
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Key dates
| Filing date | May 18, 2007 |
| Grant date | Aug 12, 2008 |
| Priority date | — |
| Expiry date | May 18, 2027 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P21/06
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to the expression and secretion in Saccharomyces cerevisiae of readily purifiable soluble variants of the Kex1 endopeptidase of Kluyveromyces lactis and the purification and use thereof for the in vitro processing of recombinant proteins usable in industrial applications. The soluble Kex1 endoproteases described here are free from the transmembrane domain of the native enzyme; the deletion of the transmembrane domain is achieved by removing at least 57 amino acid residues from the C-terminal.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.