Nucleic acid encoding fluorescent proteins from aquatic species
US7413874B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 9, 2002 |
| Grant date | Aug 19, 2008 |
| Priority date | — |
| Expiry date | Jun 1, 2024 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01K2227/40
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided are four new fluorescent proteins. The proteins were derived from two wild-type fluorescent proteins: a red fluorescent protein (RFP) that was isolated from Actinodiscus or Discosoma sp. 1 and a green fluorescent protein (GFP) isolated from Montastraea cavernosa. Two mutant forms were generated from each wild-type protein. Each of the mutated forms has a higher fluorescence intensity than the respective wild-type form. The mutant forms of the fluorescent proteins allow for more sensitive detection of the fluorescence emitted by the proteins. Additionally, one of the mutant proteins is more resistant to photobleaching than its wild-type protein. The invention also encompasses isolated nucleic acids encoding the mutant forms of the wild-type RFP and GFP.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.