Feeder-free culture method for embryonic stem cells or primate primordial stem cells
US7413902B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 24, 2002 |
| Grant date | Aug 19, 2008 |
| Priority date | — |
| Expiry date | Dec 8, 2023 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2510/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods and materials for culturing primate-derived primordial stem cells are described. In one embodiment, a cell culture medium for growing primate-derived primordial stem cells in a substantially undifferentiated state is provided which includes a low osmotic pressure, low endotoxin basic medium that is effective to support the growth of primate-derived primordial stem cells. The basic medium is combined with a nutrient serum effective to support the growth of primate-derived primordial stem cells and a substrate selected from feeder cells and an extracellular matrix component derived from feeder cells. The medium further includes non-essential amino acids, an anti-oxidant, and a first growth factor selected from nucleosides and a pyruvate salt.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.