Method for polynucleotide synthesis
US7417139B2 · kind B2 · utility
69Cited by
8References
11Claims
0Family size
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Key dates
| Filing date | Aug 30, 2003 |
| Grant date | Aug 26, 2008 |
| Priority date | — |
| Expiry date | Mar 1, 2025 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02P20/55
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Methods of forming an internucleotide bond are disclosed. Such methods find use in synthesis of polynucleotides. The method involves contacting a functionalized support with a precursor having an exocyclic amine triaryl methyl protecting group under conditions and for a time sufficient to result in internucleotide bond formation. The functionalized support includes a solid support, a triaryl methyl linker group, and a nucleoside moiety having a reactive site hydroxyl, the nucleoside moiety attached to the solid support via the triaryl methyl linker group. In particular embodiments, the precursor has the structure:wherein:
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.