On-line enzymatic digestion in separation-detection methods
US7422866B2 · kind B2 · utility
0Cited by
8References
15Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 10, 2005 |
| Grant date | Sep 9, 2008 |
| Priority date | — |
| Expiry date | Aug 10, 2025 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P21/06
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Apparatus and methods are disclosed for digesting a biopolymer in a medium. The medium is disposed adjacent a wall of a hollow element wherein at least a portion of the wall is porous. The medium is then exposed to digestion conditions to cleave the biopolymer into fragments. The fragments are permitted to permeate through the wall of the hollow element. In some embodiments a surface of a wall of the hollow element comprises a digestion agent. In some embodiments the hollow element is disposed within a hollow liner. For analysis and identification purposes, the fragments may be separated and/or detected.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.