Thermostable nucleic acid polymerase from Thermococcus gorgonarius

US7425423B1 · kind B1 · utility

Assignee

• Roche Diagnostics GMBH · DE

Inventors

• Waltraud Ankenbauer · Penzberg, DE
• Vitaly Svetlichny · Bayreuth, DE
• Elizaveta Bonch-Osmolovskaya · Moscow, RU
• Christine Ebenbichler · Antdorf, DE
• Bernhard Angerer · Rosenheim, DE
• Gudrun Schmitz-Agheguian · Bernried, DE
• Frank Laue · Kempten (Allgäu), DE

Key dates

• Filing date: Oct 1, 1997
• Grant date: Sep 16, 2008
• Expiry date: Oct 1, 2017

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/1252
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A purified thermostable enzyme is derived from the thermophilic archaebacterium Thermococcus gorgonarius. The enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95° C. in the presence of stabilizing agents and possesses 3′-5′ proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).