Site specific Listeria integration vectors and methods for using the same

US7425449B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Daniel A. Portnoy · Albany, US
• Richard Calendar · Berkeley, US
• Peter Lauer · Albany, US

Key dates

• Filing date: Apr 30, 2002
• Grant date: Sep 16, 2008
• Expiry date: Apr 30, 2022

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/975
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Site-specific Listeria integration vectors and methods for their use are provided. The subject vectors include a bacteriophage integrase gene and a bacteriophage attachment site, where in many embodiments the bacteriophage that is the source of these elements is a listeriophage. In certain embodiments, the subject vectors further include a multiple cloning site, where the multiple cloning site may further include a polypeptide coding sequence, e.g., for a heterologous antigen. The subject vectors and methods find use in a variety of different applications, including the study of Listeria species and the preparation of Listeria vaccines.