In vitro metabolic engineering on microscale devices
US7427497B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Nov 1, 2002 |
| Grant date | Sep 23, 2008 |
| Priority date | — |
| Expiry date | Jan 15, 2025 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2300/0829
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
Disclosed herein is a microfluidics device that can be used to prepare natural products and their analogs. The device comprises the enzymes of a biosynthetic pathway immobilized thereon and a means for sequentially directing a starting material and each ensuing reaction product to the enzymes of the biosynthetic pathway in the order corresponding to the steps of the biosynthetic pathway. The device can thus be used to prepare the natural product using the natural starting material of the biosynthetic pathway or analogs of the natural product using an unnatural starting material. Alternatively, artificial pathways can be created by immobilizing an appropriate selection of enzymes on the device in an order whereby each subsequent enzyme can catalyze a reaction with the product of the prior enzyme. Novel chemical entities can be prepared from these artificial pathways.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.