Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions

US7429453B2 · kind B2 · utility

Assignees

• Cornell Research Foundation, Inc. · US
• BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY AND AGRICULTURAL AND MECHANICAL COLLEGE · US
• Regents of the University of Minnesota · US

Inventors

• Francis Barany · Baltimore, US
• Matthew Lubin · Rye Brook, US
• George Barany · Falcon Heights, US
• Robert P. Hammer · Baton Rouge, US

Key dates

• Filing date: Sep 16, 2005
• Grant date: Sep 30, 2008
• Expiry date: Sep 16, 2025

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/16
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligase detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.