Methods for identifying a compound that inhibits the procession of amyloid-beta protein production in a mammalian cell expressing APP and overexpressing a G-protein coupled receptor in the cell
US7429459B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 20, 2005 |
| Grant date | Sep 30, 2008 |
| Priority date | — |
| Expiry date | Apr 20, 2025 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2800/2821
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for identifying compounds that inhibit amyloidal-beta precursor protein processing in cells, comprising contacting a test compound with a GPCR polypeptide, or fragment thereof, and measuring a compound-GPCR property related to the production of amyloidal-beta peptide. Cellular assays of the method measure indicators including second messenger and/or amyloid beta peptide levels. Therapeutic methods,and pharmaceutical compositions including effective amyloidal-beta precursor processing-inhibiting amounts of GPCR expression inhibitors, are useful for treating conditions involving cognitive impairment such as Alzheimers Disease.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.