Mutant B-type DNA polymerases exhibiting improved performance in PCR

US7429468B2 · kind B2 · utility

Assignee

• Roche Diagnostics GMBH · DE

Inventors

• Harald Sobek · Penzberg, DE
• Bruno Frey · Penzberg, DE
• Garabed Antranikian · Seevetal, DE
• Boehlke Kristina · Berlin, DE
• Francesca Maria Pisani · Roma, IT
• Mose Rossi · Napoli, IT

Key dates

• Filing date: Mar 11, 2005
• Grant date: Sep 30, 2008
• Expiry date: Apr 25, 2026

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/1252
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to thermostable mutants of B-type DNA polymerases comprising a Y-GG/A amino acid motif between the N-terminal 3′-5′-exonuclease domain and the C-terminal polymerase domain whereas the tyrosine of the Y-GG/A amino acid motif is mutated and whereas these mutant DNA polymerases are suitable for PCR.