Biological discovery using gene regulatory networks generated from multiple-disruption expression libraries
US7430475B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 26, 2002 |
| Grant date | Sep 30, 2008 |
| Priority date | — |
| Expiry date | Sep 26, 2022 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B40/00
- WIPO fieldComputer technology
- WIPO sectorElectrical engineering
Abstract
Embodiments of this invention include application of new inferential methods to analysis of complex biological information, including gene networks. In some embodiments, disruptant data and/or drug induction/inhibition data are obtained simultaneously for a number of genes in an organism. New methods include modifications of Boolean inferential methods and application of those methods to determining relationships between expressed genes in organisms. Additional new methods include modifications of Bayesian inferential methods and application of those methods to determining cause and effect relationships between expressed genes, and in some embodiments, for determining upstream effectors of regulated genes. Additional modifications of Bayesian methods include use of heterogeneous variance and different curve fitting methods, including spline functions, to improve estimation of graphs of networks of expressed genes. Other embodiments include the use of bootstrapping methods and determination of edge effects to more accurately provide network information between expressed genes. Methods of this invention were validated using information obtained from prior studies, as well as from new…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.