Isolating cells expressing secreted proteins
US7435553B2 · kind B2 · utility
54Cited by
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15Claims
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Key dates
| Filing date | May 15, 2006 |
| Grant date | Oct 14, 2008 |
| Priority date | — |
| Expiry date | May 15, 2026 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2500/10
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of detecting and isolating cells that produce any secreted protein of interest (POI), comprising: a) constructing a cell line transiently or stably expressing a cell surface capture molecule, which binds the POI, by transfecting the cell line with a nucleic acid that encodes such cell surface capture molecule; b) transfecting said cell simultaneously or subsequently with a second nucleic acid that encodes a POI wherein such POI is secreted; c) detecting the surface-displayed POI by contacting the cells with a detection molecule, which binds the POI; and d) isolating cells based on the detection molecule.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.