Beads for capturing target cells from bodily fluid
US7439062B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 23, 2004 |
| Grant date | Oct 21, 2008 |
| Priority date | — |
| Expiry date | Dec 2, 2025 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/30
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Spheroidal beads present an exterior surface of a hydrophilic hydrogel, which is an isocyanate-functional polymer that is polymerized by urethane bonds and cross-linked by urethane and urea bonds. Sequestering agents present at the surface are covalently bound to isocyanate groups or to intermediate linkers that are so bound. These beads allow sequestering agents to retain their native three-dimensional configuration, and as a result of such surface characteristics and hydrophilicity, they achieve highly effective capture of very small subpopulations of rare cells from bodily fluids or the like and very effectively deter nonspecific binding of other biomaterials present in such bodily fluid. They may be all-hydrogel spheroids or hydrogel-coated substrates.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.