Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium
US7439064B2 · kind B2 · utility
27Cited by
11References
10Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 11, 2005 |
| Grant date | Oct 21, 2008 |
| Priority date | — |
| Expiry date | Jan 13, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2501/115
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to methods for culturing human embryonic stem cells by culturing the stem cells in an environment essentially free of mammalian fetal serum and in a stem cell culture medium including amino acids, vitamins, salts, minerals, transferring, insulin, albumin, and a fibroblast growth factor that is supplied from a source other than just a feeder layer the medium. Also disclosed are compositions capable of supporting the culture and proliferation of human embryonic stem cells without the need for feeder cells or for exposure of the medium to feeder cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.