Substrates comprising polynucleotide microarrays
US7442499B2 · kind B2 · utility
5Cited by
185References
20Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 24, 1998 |
| Grant date | Oct 28, 2008 |
| Priority date | — |
| Expiry date | Nov 24, 2018 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2035/1037
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of determining the relative amounts of individual polynucleotides in a complex mixture of different-sequence polynucleotides is disclosed. The polynucleotides, after fluorescent labeling, are contacted under hybridization conditions with an array of different DNA sequences disposed at discrete locations on a non-porous surface, at an array density of at least about 100 sequences/cm2, where the different DNA sequences in the array are effective to hybridize to individual polynucleotides in the mixture. The level of fluorescence associated with each array sequence provides a measure of its relative amount in the mixture.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.