Patent · US Expired

Yeast expression vector and a method of making a recombinant protein by expression in a yeast cell

US7445775B2 · kind B2 · utility

5Cited by

3References

32Claims

0Family size

Assignee

Thromb-X n.v. · BE

Inventors

Desire Collen · Winksele, BE
Nubuo Nagai · Leuven, BE
Yves Rene LaRoche · Brussels, BE

Key dates

Filing date	Dec 20, 2001
Grant date	Nov 4, 2008
Priority date	—
Expiry date	Jan 10, 2023

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Y304/21007
WIPO fieldPharmaceuticals
WIPO sectorChemistry

Abstract

Vectors for the expression in yeast of mammalian plasminogen derivatives such as microplasminogen and miniplasminogen are presented. Methods for expression of these proteins in a methylotrophic yeast expression system are disclosed as well as the activation and stabilisation of the recombinant proteins. The proteins of this invention are used In the treatment of focal cerebral ischemic infarction and other thrombotic diseases.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.