Polypeptide for the treatment of cancer and a method for preparation thereof
US7445784B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Apr 24, 2006 |
| Grant date | Nov 4, 2008 |
| Priority date | — |
| Expiry date | Jul 19, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/71
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
This invention characterizes the specific peptide fragment derived from specially prepared zinc charged fetuin and a method of preparation thereof, wherein the fragment was found to contain an apoptosis-inducing activity. Specifically, the amino acid sequence of this peptide is His Thr Phe Ser Gly Val Ala Ser Val Glu (SEQ ID NO: 1). The peptide induced apoptosis in LNCaP (prostate cancer) and HT-29 (colon cancer) cells without affecting CCD 18 Co (normal colon) cells. The in vitro tissue culture study demonstrated that the peptide is more potent than the parent molecule (full-length zinc charged fetuin) in inducing apoptosis. The peptide has a LD50 of 0.3-0.4 μM, while the LD50 for zinc-charged fetuin is 3-10 μM.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.