Quantitative amplification with a labeled probe and 3′ to 5′ exonuclease activity
US7445898B2 · kind B2 · utility
2Cited by
28References
41Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 31, 2005 |
| Grant date | Nov 4, 2008 |
| Priority date | — |
| Expiry date | Mar 31, 2025 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6851
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention provides methods and kits for performing a quantitative amplification reaction. The method employs a polymerase enzyme and an enzyme having a 3′ to 5′ exonuclease activity that cleaves the 3′ oligonucleotide of the probe.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.