Compositions and methods for enrichment of neural stem cells using ceramide analogs
US7445931B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Sep 25, 2003 |
| Grant date | Nov 4, 2008 |
| Priority date | — |
| Expiry date | Aug 19, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2506/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides compositions and methods for human neural cell production. More particularly, the present invention provides cellular differentiation methods employing amphiphilic lipid compounds, preferably ceramide analogs of the β-hydroxyalkylamine type and optionally employing an essentially serum free MEDII conditioned medium for the generation of human neural cells from pluripotent human cells. The methods alternatively comprise modulating apoptosis by modifying the levels of PAR-4, with or without the presence of amphiphilic lipid compounds and optionally employing MEDII conditioned medium. The methods alternatively encompass modulating apoptosis by modulating the intracellular concentration of endogenous lipid second messengers, such as ceramide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.