Plasmids and utilization thereof
US7446187B2 · kind B2 · utility
4Cited by
1References
6Claims
0Family size
Assignee
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Key dates
| Filing date | Oct 29, 2004 |
| Grant date | Nov 4, 2008 |
| Priority date | — |
| Expiry date | Mar 4, 2025 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P13/001
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A shuttle vector is constructed by preparing a DNA region replicable in bacteria belonging to the genus Rhodococcus from a Rhodococcus-derived plasmid having the nucleotide sequence set forth as SEQ ID NO: 73 and a plasmid or its DNA fragment having the nucleotide sequence set forth as SEQ ID NO: 74, and a DNA region replicable in E. coli from an E. coli-derived plasmid or its DNA fragment. An aminoketone asymmetric reductase gene is inserted into the shuttle vector, transformants containing the vector are created, and the aminoketone asymmetric reductase and optically active aminoalcohols are produced.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.