Method for selecting genetically transformed cells
US7449290B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 6, 2002 |
| Grant date | Nov 11, 2008 |
| Priority date | — |
| Expiry date | Jan 8, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y302/01028
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An environmental friendly and non toxic method for selection of transformed cells from a population consisting of transformed and non-transformed cells. The method comprises the following steps a) introducing into a cell at least one desired nucleotide sequence and at least one selection-nucleotide sequence to obtain a genetically transformed cell, wherein the selection-nucleotide sequence comprises a region which codes for a protein involved in the metabolizing of trehalose; b) placing a population with transformed and non-transformed cells into contact with trehalose and/or derivative thereof; and c) selecting the transformed cells from the population on the basis of the capacity of the transformed cells to metabolize the trehalose and/or derivative.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.