Method for stabilization of proteins using non-natural amino acids
US7449443B2 · kind B2 · utility
8Cited by
1References
19Claims
0Family size
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Key dates
| Filing date | May 21, 2004 |
| Grant date | Nov 11, 2008 |
| Priority date | — |
| Expiry date | May 21, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P21/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a method for producing modified stable polypeptides introducing at least one non-natural amino acid into the hydrophobic region of the polypeptide. The thermal and chemical stability of such polypeptides is improved compared to those properties of its corresponding wild type proteins.The invention further provides purified leucine zipper and coiled-coil proteins in which the leucine residues have been replaced with 5,5,5-trifluoroleucines, and the modified proteins so produced demonstrate increased thermal and chemical stability compared to their corresponding wild-type natural proteins.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.