Site-specific labelling of proteins
US7449558B2 · kind B2 · utility
71Cited by
1References
12Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 29, 2004 |
| Grant date | Nov 11, 2008 |
| Priority date | — |
| Expiry date | Aug 5, 2025 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/58
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An in vivo method for labelling a protein having an N-terminal cysteine is disclosed. A probe comprising a thioester group and a detectable label is introduced into a cell expressing the N-terminal cysteine protein, allowing for the N-terminal cysteine to cleave the thioester bond and resulting in the label being covalently attached to the N-terminus of the protein by an amide bond.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.