Patent · US Expired

Signal sequences for preparing leu-hirudin by secretion by E. coli into the culture medium

US7455987B1 · kind B1 · utility

4Cited by

7References

4Claims

0Family size

Assignee

Sanofi-Aventis Deutschland GMBH · DE

Inventors

Paul Habermann · Solms, DE
Rudolf Bender · Kronberg, DE

Key dates

Filing date	Sep 18, 2000
Grant date	Nov 25, 2008
Priority date	—
Expiry date	Sep 25, 2022

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K2319/02
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention relates to a efficient expression of a protein, particularly, hirudin; a protein precursor comprising a signal sequence and the sequence of aaX-hirudin, wherein aaX is a selected amino acid, and aaX is preferably leucine; to its preparation and use; and to processes for finding signal sequences for secretory expression of any desired protein in E coli; and to processes for the secretory expression of any desired protein in E coli.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.