Device and method for providing a hybridization chamber and for influencing air bubbles in the same
US7459306B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 2, 2004 |
| Grant date | Dec 2, 2008 |
| Priority date | — |
| Expiry date | Feb 23, 2026 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2400/086
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
A device, appropriate process units and/or systems for providing a gap-shaped hybridization space to hybridize nucleic acid samples, proteins or tissue sections over a slide. Said items of device are able to move in relation to the slide and comprise an annular sealing surface to seal the hybridization space by application to a surface of the slide. Said items of device additionally comprise lines to introduce media to and remove them from the hybridization space. Said items of device furthermore comprise a specimen supply line and preferably an agitation device to move fluids in relation to the samples immobilized on the surface of the slide. The devices according to the invention are characterized in that they limit a hybridization space that features relief structures to conduct and/or block air bubbles on at least one surface of the device and the slide directed at the interior of this hybridization space. According to the method of the invention, air bubbles present in the hybridization space are conducted and/or blocked by these relief structures.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.