Patent · US Expired

Fluorescent label compounds

US7465747B2 · kind B2 · utility

4Cited by
0References
29Claims
0Family size

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Key dates

Filing dateMar 10, 2003
Grant dateDec 16, 2008
Priority date
Expiry dateNov 11, 2024

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG01N33/533
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

It is intended to provide novel labeling reagents characterized by having a group capable of binding to a substance to be labeled (for example, a biological substance, a physiologically active substance, etc.), easily forming a complex together with a rare earth ion, the complex being stable in an aqueous solution, and having a sufficient fluorescence intensity and a long fluorescence life time regardless of buffer types; complexes composed of the above labeling reagent with a rare earth ion; fluorescence labels containing the above complex; a fluorescence assay method using the above fluorescent label; etc. Namely, labeling reagents comprising a compound having a 2,2′:6′,2″-tripyridine skeleton or a 2,6-dipyrazolopyridine skeleton and having a group capable of binding to a substance to be labeled (for example, a biological substance, a physiologically active substance, etc.) and a group capable of forming a complex together with a rare earth ion; complexes composed of the above labeling reagent with a rare earth ion; fluorescence labels containing the above complex; a fluorescence labeling method using the above complex as a label; and a fluorescence assay method using the above f…

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