Yeast cell surface display of proteins and uses thereof
US7465787B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 16, 2003 |
| Grant date | Dec 16, 2008 |
| Priority date | — |
| Expiry date | Dec 16, 2023 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/39
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides a genetic method for tethering polypeptides to the yeast cell wall in a form accessible for binding to macromolecules. Combining this method with fluorescence-activated cell sorting provides a means of selecting proteins with increased or decreased affinity for another molecule, altered specificity, or conditional binding. Also provided is a method for genetic fusion of the N terminus of a polypeptide of interest to the C-terminus of the yeast Aga2p cell wall protein. The outer wall of each yeast cell can display approximately 10 protein agglutinins. The native agglutinins serve as specific adhesion contacts to fuse yeast cells of opposite mating type during mating. In effect, yeast has evolved a platform for protein-protein binding without steric hindrance from cell wall components.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.