Compositions and methods for altering the disulfide status of proteins

US7465852B2 · kind B2 · utility

Assignees

• PIONEER HI-BRED INTERNATIONAL, INC. · US
• E.I. DU PONT DE NEMOURS AND COMPANY · US

Inventors

• Vincent Sewalt · West Des Moines, US
• Craig Hastings · Perry, US
• Robert Meeley · Des Moines, US
• Sabine Hantke · Cologne, DE
• Rudolf Jung · Des Moines, US
• John D. Everard · Wilmington, US
• Stephen M. Allen · Chandler, US

Key dates

• Filing date: Aug 2, 2005
• Grant date: Dec 16, 2008
• Expiry date: Jun 6, 2026

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A23V2002/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Compositions and methods for the alteration of the disulfide status of plant proteins are provided. Novel nucleotide molecules, comprising nucleotide sequences for thioredoxin h and NADPH-thioredoxin reductase, and the proteins encoded thereby are provided. The nucleotide sequences can be used to transform plants alone or in combination with other sequences, such as sequences encoding NADPH generating enzymes, to manipulate the thioredoxin h system and alter the protein disulfide status in plants. Transformed plants, plant cells, plant tissues, seed and grain are provided.