Enriched preparation of human fetal multipotential neural stem cells
US7468277B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Dec 22, 2000 |
| Grant date | Dec 23, 2008 |
| Priority date | — |
| Expiry date | Dec 22, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2840/44
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention relates to a method of separating multipotential neural progenitor cells from a mixed population of cell types. This method includes selecting a promoter which functions selectively in the neural progenitor cells, introducing a nucleic acid molecule encoding a fluorescent protein under control of said promoter into all cell types of the mixed population of cell types, allowing only the neural progenitor cells, but not other cell types, within the mixed population to express said fluorescent protein, identifying cells of the mixed population of cell types that are fluorescent, which are restricted to the neural progenitor cells, and separating the fluorescent cells from the mixed population of cell types, wherein the separated cells are restricted to the neural progenitor cells. The present invention also relates to an isolated human musashi promoter and an enriched preparation of isolated multipotential neural progenitor cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.