Methods for selecting a yeast population for the production of an organic acid and producing an organic acid
US7473540B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 20, 2006 |
| Grant date | Jan 6, 2009 |
| Priority date | — |
| Expiry date | May 24, 2027 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/395
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of producing an organic acid by staining a yeast population with a stain capable of internal pH (pHi)-dependent fluorescence, to yield a stained yeast population; determining a gate pH and a corresponding fluorescence parameter of the stained yeast population; and sorting the cells of the stained yeast population such that the cells having a pHi above the gate pH are retained and the cells having a pHi below the gate pH are discarded, to yield a yeast population for the production of the organic acid. Also, a method of producing an organic acid by performing the above steps, followed by isolating individual cells of the yeast population, to yield individual yeast cells for the production of an organic acid; culturing an individual yeast cell, to yield a cloned yeast population for the production of an organic acid; and incubating the cloned yeast population for the production of an organic acid in a medium containing an organic acid precursor, to produce the organic acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.