Dual illumination system for an imaging apparatus and method
US7474399B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 15, 2006 |
| Grant date | Jan 6, 2009 |
| Priority date | — |
| Expiry date | Apr 15, 2027 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2201/0826
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A dual illumination system is disclosed for use with an imaging apparatus. The imaging apparatus defines a light-tight imaging compartment with an interior wall having a view port extending into the imaging compartment. This view port enables data acquisition of a biological specimen contained in the imaging compartment. The dual illumination system includes a first illumination assembly configured to direct structured light onto a first side of the specimen to enable structured light and surface topography measurements thereof. A second illumination assembly then directs light at the specimen wherein diffused fluorescent light emanates from a surface thereof for receipt through the view port to acquire fluorescence data of the specimen. The combination of structured light imaging and fluorescence imaging enables 3D diffuse tomographic reconstructions of fluorescent probe location and concentration.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.