Affinity trap reactor and single-step process for purifying angiostatin-like fragment from human plasma using the same
US7476515B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 17, 2003 |
| Grant date | Jan 13, 2009 |
| Priority date | — |
| Expiry date | Jul 29, 2023 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y304/21007
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides an affinity trap reactor that enables the reaction between an enzyme bound to a support and substrate to proceed efficiently without its applications being restricted by the type of enzyme and substrate used. The present invention relates to an affinity trap reactor composed of a support bound with an enzyme and a molecule that specifically binds with a substrate of the enzyme, and a single-stage process for obtaining BL-angiostatin from plasminogen contained in a biological sample, wherein a biological sample containing plasminogen is applied to an affinity trap reactor composed of a support bound with bacillolysin MA and lysine, and reacted under conditions of a temperature of 0 to 50° C. in the presence of isopropyl alcohol but in the absence of calcium ions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.