Nucleic acids encoding fluorescent proteins and methods of using the same
US7476518B2 · kind B2 · utility
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16Claims
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Key dates
| Filing date | Aug 19, 2005 |
| Grant date | Jan 13, 2009 |
| Priority date | — |
| Expiry date | Aug 23, 2025 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A GFP with an F64L mutation and E222G mutation is provided. This GFP has a bigger Stokes shift compared to other GFPs making it very suitable for high throughput screening due to a better resolution. This GFP also has an excitation maximum between the yellow GFP and the cyan GFP allowing for clearer band separation when used together with those GFPs. Examples include the sequences in SEQ ID NOs: 3 and 4.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.