Method of quickly detecting and/or assaying antigen by fluorescence correlation spectrometry
US7476545B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 4, 2006 |
| Grant date | Jan 13, 2009 |
| Priority date | — |
| Expiry date | Dec 4, 2026 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2800/2828
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides a method of quickly and accurately detecting and/or assaying an antigen using fluorescence correlation spectroscopy (FCS), which involves a fluorescence-labeled antibody fragment and a non-fluorescence-labeled intact antibody that form a complex with the antigen. There is a significant difference in diffusion rate between the fluorescence-labeled antibody fragment not bonded to the antigen and the complex formed by the the fluorescence-labeled antibody fragment, the antigen, and the non-fluorescence-labeled intact antibody, and this diffusion rate can be determined using FCS. The antigen can be an antigenic protein, such as an abnormal prion or a harmful protein contained in a food material. According to this method, antigens over a wide scope can be assayed regardless of the shape or molecular weight.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.